O Estresse salino na produção de exopolissacarídeos pela Chaetoceros neogracilis
DOI:
10.46551/ruc.v27n1a4Resumo
Objetivo: Avaliar o efeito da salinidade no cultivo da microalga Chaetoceros neogracilis para produção de pigmentos fotossintetizantes e exopolissacarídeos. Método: Foram realizados sete tratamentos com diferentes salinidades, por sete dias. Diariamente foram realizadas contagens celular e medição da absorbância, para analisar o crescimento e a densidade celular. Os pigmentos fotossintéticos foram determinados por espectrofotometria, e os exopolissacarídeos por precipitação alcoólica do sobrenadante. Resultados: Na salinidade 25‰ ocorreu o maior valor médio de absorbância (1.032 ± 0.039 nm), porém a maior densidade celular foi obtida na salinidade 20‰ (481±29 x104cél.mL-1). A maior concentração de pigmentos foi registrado em salinidade de 15‰ (1,820, 2,566 e 3,915 mg.L-1, para clorofila-a, feofitina-a e carotenoides respectivamente). A produção de exopolissacarídeos foi diretamente influenciada pela salinidade, com maior concentração na salinidade de 35‰ (2.952±0,204 g.L-1). Conclusão: A microalga C. neogracilis é capaz de ser produzida em uma ampla faixa de salinidades, mas para maximizar a produção de exopolissacarídeos, deve-se utilizar salinidades elevadas.
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