Canine Babesiosis: Hematological Aspects and Comparison of Diagnostic Methods

Authors

DOI:

10.46551/ruc.v23n1a02

Keywords:

28S rDNA; Blood Count; Hemoparasitosis; Babesiosis; Molecular diagnosis.

Abstract

Canine babesiosis is a hemoparasitosis caused by etiological agents of the Babesia genus. The use of clinical diagnosis is imprecise due to the existence of unspecific clinical signs, and routinely used tests such as the blood smear have many false negative results. Objective comparison of diagnostic efficiency by hematological patterns, blood smear, and by polymerase chain reaction (PCR). Method this is a research carried out with 80 dogs that were under the responsibility of the Zoonoses Control Center. By collecting 3 ml of venous blood per animal for testing. Results, there was mild to moderate anemia in the 80 samples analyzed, but without significant differences in the blood count parameters of dogs positive and negative for babesiosis. Although the diagnosis by blood smear was negative, PCR was 46.2% (37/80) positive for Babesia canis. A high rate of positive results for Babesia canis was identified in dogs parasitized by the vector tick. Final considerations from the
results found, it is concluded that there is weakness in the tests routinely used for the diagnosis of canine babesiosis when compared to molecular diagnosis. Evidencing the need to implement new methodologies for more efficient diagnosis. 

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Author Biographies

Eloan Mendes Vieira, Universidade Estadual de Montes Claros - Unimontes

Mestre em biotecnologia Universidade Estadual de Montes Claros (UNIMONTES). Montes Claros/MGBrasil. Doutorando da Universidade Federal de Ouro Preto (UFOP). Ouro Preto/MG. Brasil. 

Júlia Rodrigues Ortega, Universidade Estadual de Montes Claros - Unimontes

Mestranda em Biotecnologia pela Universidade Estadual de Montes Claros (UNIMONTES). Montes
Claros/MG- Brasil. 

Vanessa de Andrade Royo, Universidade Estadual de Montes Claros - Unimontes

Doutora em produtos naturais sintéticos. Universidade de São Paulo, USP. Professor Departamento de Ciências Biológicas, Universidade Estadual de Montes Claros (UNIMONTES). Montes Claros/MG- Brasil. 

Afrânio Farias de Melo Júnior, Universidade Estadual de Montes Claros - Unimontes

Doutor em Engenharia Florestal. Universidade Federal de Lavras, Professor Departamento de Ciências
Biológicas, Universidade Estadual de Montes Claros – UNIMONTES, Montes Claros MG- Brasil.

Dario Alves de Oliveira, Universidade Estadual de Montes Claros - Unimontes

Doutor em Fitotecnia (Produção Vegetal) pela Universidade Federal de Viçosa, UFV, Brasil. Professor do
Departamento de Ciências Biológicas da Universidade Estadual de Montes Claros – UNIMONTES, Montes
Claros/MG. Brasil. 

Elytania Veiga Menezes, Universidade Estadual de Montes Claros - Unimontes

Doutora em Ciências Biológicas (Biologia Genética). Universidade de São Paulo, USP, Brasil. Professor do
Departamento de Ciências Biológicas da Universidade Estadual de Montes Claros – UNIMONTES, Montes
Claros/MG – Brasil. 

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Published

2021-06-18

How to Cite

VIEIRA, Eloan Mendes; ORTEGA, Júlia Rodrigues; ROYO, Vanessa de Andrade; JÚNIOR, Afrânio Farias de Melo; OLIVEIRA, Dario Alves de; MENEZES, Elytania Veiga. Canine Babesiosis: Hematological Aspects and Comparison of Diagnostic Methods. Revista Unimontes Científica, [s. l.], vol. 23, no. 1, p. 1–16, 2021. DOI: 10.46551/ruc.v23n1a02. Disponível em: https://www.periodicos.unimontes.br/index.php/unicientifica/article/view/4309. Acesso em: 22 jul. 2024.

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