Comparative Analysis of Coffea Arabica Honeys from Two Locations in Minas Gerais
DOI:
10.46551/ruc.v26n2a2Keywords:
Antioxidant activity, caffeine, phenolics compounds, honey, physico-chemical analysisAbstract
Objective: To perform physicochemical analyses, pollen count, evaluation of antioxidant activity, and quantification of caffeine in honeys derived from the flowering of Coffea arabica, originating from two distinct locations: M1 (Santana da Vargem) and M2 (Felício dos Santos). Methodology: For the analyses, tests for botanical identification, Fiehe reaction, Lund test, diastase enzymatic test, dye test, presence of dextrins test, color classification, acidity determination, moisture determination, determination of hydroxymethylfurfural, determination of reducing sugars, determination of insoluble solids, ash content determination, apparent sucrose, total polyphenols, antioxidant activity analysis, and caffeine quantification were performed. Results: It was possible to observe that only the hydroxymethylfurfural content of sample M2 is above the allowed limit, the other analyses are within the limits established by normative instruction no. 11 of 10/20/2000, besides high levels of polyphenols, and similar caffeine dosage for both honeys. Conclusion: The differences observed between the honeys are likely related to the climate and region in which they were produced. Regarding antioxidant activity, the best result was found for M1; however, higher levels of polyphenols and caffeine were found in M2.
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KOH, Ilyun et al. Climate risks to Brazilian coffee production. Environmental Research Letters, v. 15, n. 10, p. 104015, 2020. https://doi.org/10.1088/1748-9326/aba471
RIBEIRO, Juliano Ribeiro et al. The use of chromatographic profiles from roasted arabica coffees to differentiate samples according to cleanliness, flavour and overall quality of the beverage. Química Nova, v. 33, p. 1897-1904, 2010. https://doi.org/10.1590/S0100-40422010000900015
SIMÕES, Juliana Carvalho; PELEGRINI, Djalma Ferreira. Diagnóstico da cafeicultura mineira–1: regiões tradicionais–Sul/Sudoeste de Minas, Zona da Mata, Triângulo Mineiro/Alto Paranaíba. Belo Horizonte: EPAMIG, 2010.
SENINDE, Denis Richard; CHAMBERS, Edgar Coffee Flavor: A Review. Beverages, v. 6, n. 3, p. 44, 2020. https://doi.org/10.3390/beverages6030044
KLEIN, Alexandra-Maria et al. Importance of pollinators in changing landscapes for world crops. Proceedings of the royal society B: biological sciences, v. 274, n. 1608, p. 303-313, 2007. https://doi.org/10.1098/rspb.2006.3721
MARLEBO- SOUZA, Darclet Teresinha. The corn pollen as a food source for honeybees. Acta Scientiarum. Agronomy, v. 33, p. 701-704, 2011. https://doi.org/10.4025/actasciagron.v33i4.10533
AMARAL, Érico Marcelo Hoff do Amaral et al. The role of insects in the pollination of caturra coffee. Revista de Agricultura, Piracicaba, v. 35, p. 139-47, 1960.
MELO, Adriane Alexandre Machado et al. Composition and properties of Apis mellifera honey: A review. Journal of apicultural research, v. 57, n. 1, p. 5-37, 2018. https://doi.org/10.1080/00218839.2017.1338444
HOSSAIN, Md Lokman et al. Monitoring the Release of Methylglyoxal (MGO) from Honey and Honey-Based Formulations. Molecules, v. 28, n. 6, p. 2858, 2023. DOI 10.3390/molecules28062858.
MURADIAN, Ligia Bicudo de Almeida et al. Standard methods for Apis mellifera honey research. Journal of Apicultural Research, v. 59, n. 3, p. 1-62, 2020.https://doi.org/10.1080/00218839.2020.1738135
NZEKOUE, Franks Kamgang et al. Quantification of 30 Bioactive Compounds by a New HPLC-MS/MS Method and Evaluation of Their Antioxidant and Antibacterial Activities. Food Research International, v. 133, p. 109128, 2020. https://doi.org/10.1016/j.foodres.2020.109128
RACOWSKI, Ilana. et al. Ação antimicrobiana do mel em leite fermentado. Revista Analytica, v. 30, p. 115-117, 2007. Acesso em: https://www.yumpu.com/pt/document/read/10591564/acao-antimicrobiana-do-mel-em-leite-fermentado-revista-analytica
LOUVEAUX, Jérôme, MAURIZIO, Anna and VORWOHL, G. Methods of Melissopalynology. Bee World, v. 59, n. 4, p. 139-157, 1978.
BARTH, Ortrud Monika. O pólen no mel brasileiro. Rio de Janeiro: Instituto Oswaldo Cruz, 1989. Disponível online: https://www.researchgate.net/publication/311946380_O_Polen_no_Mel_Brasileiro
BRASIL. Ministerio da Agricultura e Abastecimento. Instrução Normativa n° 11, de 20 de Outubro de 2000. Diario Oficial da União. 23 de outubro de 2000. Seção 1, p. 23.
SOARES, Sónia et al. A comprehensive review on the main honey authentication issues: Production and origin. Comprehensive Reviews in Food Science and Food Safety, v. 16, n. 5, p. 1072-1100, 2017.https://doi.org/10.1111/1541-4337.12278
SEAPA. Exportação de mel cresce em Minas Gerais. Agricultura.mg.gov.br. 2011. Disponível em: http://www.agricultura.mg.gov.br/index.php/institucional/55-conteudo/noticias/2230-exportacao-de-mel-cresce-em-minas-gerais.
BRASIL, Ministério da Agricultura, Secretaria de Defesa agropecuária, Departamento de inspeção de produtos de origem animal – DIPOA. Instrução Normativa Nº 11, de 20 de Outubro de 2000.
GUTTENTAG, Annabel; KRISHNAKUMAR, Krishothman; COKCETIN, Nural; HARRY, Elizabeth; CARTER, Dee. Factors affecting the production and measurement of hydrogen peroxide in honey samples. Access Microbiology. Mar. 2021. DOI 10.1099/acmi.0.000198.
AOAC (Association Of Official Analytical Council). Official methods of Analysis. Arlington: AOAC, 1998.
CAC (Codex Alimentarius Commission). Official Methods of Analysis, v.3, supl.2, p.15-39, 1990.
IAF (Instituto Adolfo Lutz). Métodos Físico-Químicos para Análise de Alimentos - 4ª Edição. 2008.
SINGLETON, Vernon; ORTHOFER, Rudolf; LAMUELA-RAVENTÓS, Rosa. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: 7. Academic press, 1999. p. 152-178. http://dx.doi.org/10.1016/S0076-6879(99)99017-1
BASTOS, Esther Margarida Alves Ferreira et al. Characterization of the honey from Myracrodruon urundeuva (Anacardiceae-Aroeira) in the Dry Forest of northern of Minas Gerais/Brazil. Advances in Agricultural Science, v. 4, n. 4, p. 64-71, 2016.
BRAND-WILLIAMS, Wendy; CUVELIER, Marie-Elisabeth; BERSET, Claudette. Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, v. 28, n. 1, p. 25-30, 1995. https://doi.org/10.1016/S0023-6438(95)80008-5
PENA JÚNIOR, Deosvaldo et al. Antioxidant activities of some monofloral honey types produced across Minas Gerais (Brazil). Plos One, v. 17, n. 1, p. e0262038, 2022. https://doi.org/10.1371/journal.pone.0262038
RUFINO, Maria do Socorro Moura et al. Metodologia científica: determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH. Embrapa, 2007.
DIAS, José Santos. Physicochemical Characterization of Honey Samples. UNOPAR Científica Ciências Exatas e Tecnológicas, Londrina, v. 8, p. 19-22, 2009.
BRAGA, Dayana Calixto et al. Analytical study of the physicochemical characteristics from Melipona subnitida D. honey in adequation to Brazilian law. Food Science and Technology, v. 40, p. 217-221, 2019.https://doi.org/10.1590/fst.08919
ABADIO FINCO, Fernanda Dias Bartolomeu; MOURA, Luciana Learte; SILVA, Igor Galvão. Propriedades físicas e químicas do mel de Apis mellifera L. Food Science and Technology, v. 30, p. 706-712, 2010. doi 10.1590/s0101-20612010000300022.
RAZALI, Muhammad Faiz et al. Effect of high-pressure processing (hpp) on antioxidant, diastase activity and colour for Kelulut (stingless bee) honey. Journal Technology, v. 81, p. 91-98, 2019. doi 10.11113/jt.v81.13105.
OLIVEIRA, Melissa Dos Santos; PLETSCH, Lidia Betina Hendges; MARQUES, Marieli da Silva. Avaliação da qualidade do mel comercializado na Região Celeiro do RS. 12 Simpósio Latino-Americano de Ciências de Alimentos, 2017.
HAOUAM, Lynda et al. The quality of honeys influenced by the traditional heating method. The Journal of Microbiology, Biotechnology and Food Sciences, v. 8, n. 6, p. 1276, 2019. doi: 10.15414/jmbfs.2019.8.6.1276-1280
WARUI, Mary Wanjiru et al. Characterization of Kenyan honeys based on their physicochemical properties, botanical and geographical origin. International Journal of Food Science, v. 2019, n. 4, 2019. https://doi.org/10.1155/2019/2932509
SOLAYMAN, Md et al. Physicochemical properties, minerals, trace elements, and heavy metals in honey of different origins: a comprehensive review. Comprehensive Reviews in Food Science and Food Safety, v. 15, n. 1, p. 219-233, 2016. https://doi.org/10.1111/1541-4337.12182
GOMES, Victor Valentim et al. Avaliação da qualidade do mel comercializado no oeste do Pará, Brasil. Revista Virtual de Química, v. 9, n. 2, p. 815-826, 2017. doi: 10.21577/1984-6835.20170050
LACERDA, Julian Júnio de Jesus et al. Influência das características físico-químicas e composição elementar nas cores de méis produzidos por Apis mellifera no sudoeste da Bahia utilizando análise multivariada. Química Nova, v. 33, p. 1022-1026, 2010. https://doi.org/10.1590/S0100-40422010000500003
PONTIS, Jonierison Alves et al. Color, phenolic and flavonoid content, and antioxidant activity of honey from Roraima, Brazil. Food Science and Technology, v. 34, p. 69-73, 2014. doi 10.1590/s0101-20612014005000015.
BERETTA, Giangiacomo et al. Standardization of antioxidant properties of honey by a combination of spectrophotometric/fluorimetric assays and chemometrics. Analytica Chimica Acta, v. 533, n. 2, p. 185-191, 2005. 10.1016/j.aca.2004.11.010.
FERREIRA, Isabel Cristina Fernandes Rodrigues et al. Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic extract. Food chemistry, v. 114, n. 4, p. 1438-1443, 2009. doi 10.1016/j.foodchem.2008.11.028.
KAŠKONIENĖ, Vilma et al. Quantitative and qualitative determination of phenolic compounds in honey. Cheminė technologija, v. 52, n. 3, p. 74-80, 2009.
SILVA, Priscila Mission et al. Honey: Chemical composition, stabilityand authenticity. Food Chemistry., v.196, p.309–323, 2016. doi:10.1016/j.foodchem.2015.09.051
SINGH, Iqbal; SINGH, Sukhmeet. Honey moisture reduction and its quality. Journal of food science and technology, v. 55, p. 3861-3871, 2018. https://doi.org/10.1007/s13197-018-3341-5
SHAPLA, Ummay Mahfuza et al. 5-Hydroxymethylfurfural (HMF) levels in honey and other food products: effects on bees and human health. Chemistry Central Journal, v. 12, n. 1, p. 1-18, 2018. https://doi.org/10.1186/s13065-018-0408-3
ROYO, Vanessa de Andrade et al. Physicochemical Profile, Antioxidant and Antimicrobial Activities of Honeys Produced in Minas Gerais (Brazil). Antibiotics, v. 11, n. 10, p. 1429, 2022. https://doi.org/10.3390/antibiotics11101429
SCHIASSI, Maria Cecília Evangelista Vasconcelos et al. Quality of honeys from different botanical origins. Journal of Food Science and Technology, v. 58, p. 4167-4177, 2021. doi 10.1007/s13197-020-04884-7.
ARCHILIA, Mariana Degaki et al. Characterization of Brazilian monofloral and polyfloral honey by UHPLC-MS and classic physical-chemical analyses. Journal of Apicultural Research, v. 62, n. 3, p. 578-589, 2023. doi 10.1080/00218839.2021.1886747.
TRINH, Nguyen Thi Nu et al. Chemical composition analysis and antioxidant activity of coffea robusta monofloral honeys from Vietnam. Foods, v. 11, n. 3, p. 388, 2022. doi 10.3390/foods11030388.
DEBELA, Hawine; BELAY, Abera. Caffeine, invertase enzyme and triangle test sensory panel used to differentiate Coffea arabica and Vernonia amygdalina honey. Food Control, v. 123, p. 107857, 2021. doi 10.1016/j.foodcont.2020.107857.
CAC (Codex Alimentarius Commission Standards). Revised Codex Standards for Honey; Codex Standard 12–1981, Rev. 2; Joint FAO/WHO Food Standards Programme. 24th Session; FAO Headquarters: Rome, Italy, 2001
ZAMORA, María Clara; CHIRIFE, Jorge. Determination of water activity change due to crystallization in honeys from Argentina. Food Control, v. 17, n. 1, p. 59-64, 2006. https://doi.org/10.1016/j.foodcont.2004.09.003.
KUROISHI, Alini Mari et al. Evaluation of honey crystallization from the colour and water activity parameters. Brazilian Journal of Food Technology, v. 15, p. 84-91, 2012. https://doi.org/10.1590/S1981-67232012000100009
FONSECA, Antônio Augusto et al. Qualidade do mel de abelhas sem ferrão: uma proposta para boas práticas de fabricação. Universidade Federal do Recôncavo da Bahia: SECTI-FAPESB, 2006.
NORA, Adri; WILAPANGGA, Anjas; NOVIANTI, Titta. Antioxidant activity, antibacterial activity, water content, and ash content in Baduy Honey. Bioscience, v. 2, n. 1, p. 38-44, 2018.https://doi.org/10.24036/0201822101249-0-00
AZONWADE, François Ezin et al. Physicochemical characteristics and microbiological quality of honey produced in Benin. Journal of Food Quality, v. 2018, p. 1-13, 2018. https://doi.org/10.1155/2018/1896057
ADDI, Admassu et al. Physicochemical and antioxidant properties of Coffea arabica honey from Western Oromia, Ethiopia. International Journal of Agricultural Science and Food Technology, v. 8, n. 2, p. 159-165, 2022. https://doi.org/10.17352/2455-815x.000158
MARCOLIN, Lucas Cavagnoli et al. Meliponinae and Apis mellifera honey in southern Brazil: Physicochemical characterization and determination of pesticides. Food Chemistry, v. 363, p. 130175, 2021. https://doi.org/10.1016/j.foodchem.2021.130175
CRĂCIUN, Mihaela Emanuela et al. Characterization and classification of Romanian acacia honey based on its physicochemical parameters and chemometrics. Scientific Reports, v. 10, n. 1, p. 20690, 2020. https://doi.org/10.1038/s41598-020-77685-9
FARHAT, Naima et al. Dietary phenolic compounds in plants: Their antioxidant and pharmacological potential. Postepy Biologii Komorki, v. 47, n. 3, p. 307-20, 2020.
LACHMAN, Jaromír et al. Evaluation of antioxidant activity and total phenolics of selected Czech honeys. LWT-Food Science and Technology, v. 43, n. 1, p. 52-58, 2010. https://doi.org/10.1016/j.lwt.2009.06.008
HUSSEIN, Saba Zuhair et al. Antioxidant capacities and total phenolic contents increase with gamma irradiation in two types of Malaysian honey. Molecules, v. 16, n. 8, p. 6378-6395, 2011. https://doi.org/10.3390/molecules16086378
OLIVEIRA, Fernanda Moreira et al. Classificação de méis do Rio Grande do Sul (Brasil) pela análise multivariada de dados com base nas propriedades físicas e composição química. Brazilian Journal of Development, v. 6, n. 8, p. 57413-57431, 2020. https://doi.org/10.34117/bjdv6n8-231
ANDRADE, Beatriz Becevelli et al. Mel de abelhas sem ferrão: uma revisão sobre parâmetros químicos, teor de compostos bioativos e suas propriedades terapêuticas. Research, Society and Development, v. 11, n. 16, p. e77111637618-e77111637618, 2022. https://doi.org/10.33448/rsd-v11i16.37618
ZHANG, Yu-Jie et al. Antioxidant phytochemicals for the prevention and treatment of chronic diseases. Molecules, v. 20, n. 12, p. 21138-21156, 2015. https://doi.org/10.3390/molecules201219753
BASTOS, Deborah Helena Markowicz; ROGERO, Marcelo Macedo.; ARÊAS, José Alfredo Gomes. Mecanismos de ação de compostos bioativos dos alimentos no contexto de processos inflamatórios relacionados à obesidade. Arquivos Brasileiros de Endocrinologia & Metabologia, v. 53, p. 646-656, 2009. https://doi.org/10.1590/S0004-27302009000500017
MARTINS, Natália; PETROPOULOS, Spyridon; FERREIRA, Isabel Cristina Fernandes Rodrigues. Chemical composition and bioactive compounds of garlic (Allium sativum L.) as affected by pre-and post-harvest conditions: A review. Food Chemistry, v. 211, p. 41-50, 2016. doi: 10.1016/j.foodchem.2016.05.029.
OROIAN, Mircea; ESCRICHE, Isabel. Antioxidants: Characterization, natural sources, extraction and analysis. Food Research International, v. 74, p. 10-36, 2015. https://doi.org/ 10.1016/j.foodres.2015.04.018.
SHAHIDI, Fereidoon; AMBIGAIPALAN, Priyatharini. Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects–A review. Journal of functional foods, v. 18, p. 820-897, 2015. https://doi.org/10.1016/j.jff.2015.06.018
SINDHI, Vinita et al. Potential applications of antioxidants–A review. Journal of pharmacy research, v. 7, n. 9, p. 828-835, 2013. https://doi.org/10.1016/j.sjbs.2018.08.010
EMBUSCADO, Milda. Spices and herbs: Natural sources of antioxidants–a mini review. Journal of Functional Foods, v. 18, p. 811-819, 2015. https://doi.org/10.1016/j.jff.2015.03.005
SHAHIDI, Fereidoon; ZHONG, Ying. Measurement of antioxidant activity. Journal of Functional Foods, v. 18, p. 757-781, 2015. https://doi.org/10.1016/j.jff.2015.01.047
DE MARIA, Carlos Alberto Bastos; MOREIRA, Ricardo Felipe Alves. Métodos para análise de ácido clorogênico. Química Nova, v. 27, p. 586-592, 2004. https://doi.org/10.1590/S0100-40422004000400013
KADRI, Samir Moura et al. Characterization of Coffea arabica monofloral honey from Espírito Santo, Brazil. Food Chemistry, v. 203, p. 252-257, 2016. https://doi.org/10.1016/j.foodchem.2016.02.074
HUXTABLE, Ryan James. The toxicology of alkaloids in foods and herbs. In: Handbook of natural toxins. Routledge, 2019. p. 237-262.
